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HPLC - electrochemical assay for monitoring the formation of 8-oxo-7,8-dihydroadenine and its related 2-deoxyribonucleoside
Summary
Type:
HPLC
Application #:
107040
Catalog#:
720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection:
amperometric, glassy carbon electrodes at 850 and 650mV
Conditions:
eluent: 50mM Na citrate pH 5.0 - methanol (87.5:12.5)
flow rate 1ml/min
Authors:
Berger M
Source:
J Liquid Chromatogr 13 (1990) 929 - 940
Details
Legend: Fig. 2. HPLC profile of 8-hydroxylated derivatives of adenine and guanine components, 1. 8-oxo-7,8-dihydroguanine, 2. 8-oxo-7,8-dihydro-2' -dixocyguanosine, 3. 8-oxo-7,8-dihydro-adenine, 4. 8-oxo-7,
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